Teenage winger Moses had been struggling with a thigh injury and lasted just 16 minutes of Saturday's 1-1 draw at Cardiff before having to be substituted.
Midfielder Ambrose is also doubtful for the Walkers Stadium clash after appearing to suffer a pulled hamstring against the Bluebirds.
Freddie Sears came on for Moses to prove his fitness after a month out with a sprained ankle and fellow striker Stern John got a late run-out as a substitute for his first appearance since dislocating his elbow on the opening day of the season.
Centre-half Claude Davis will hope to be involved after illness while left-back Clint Hill played at the weekend despite a virus and should keep his place.
Source: Team_Talk