Having studied video evidence, Forest initially announced they were planning to appeal the decision.
However, upon further examination of the incident between Chambers and Reading's Shane Long in the 0-0 draw at the Madejski Stadium on Saturday, the club have decided to take no further action.
The decision gives manager Billy Davies a further selection headache with fellow defenders Kelvin Wilson, James Perch and Julian Bennett already set for lengthy spells on the sidelines through injury.
Source: Team_Talk