Wycombe spent a total of £29,660 on agents` fees last season.
League One clubs paid out a combined total of £2,241,771 during the period from July 1 2009 to June 30 2010 with Southampton the biggest spenders, splashing out £401,248.
Spending on agents' fees increased by £3.9million across the whole of the Football League last year.
On announcing the latest figures, Football League chairman Greg Clarke said: "Given the current economic climate, it is worrying to see such a significant amount leaking from the game.
"This year's figures demonstrate a considerable increase on those of last season and it is essential that clubs work to reduce this liability over the coming campaign."