The Premier League have fined Wolves £25,000 for fielding a weakened team in the 3-0 defeat at Manchester United in mid-December.
Wolves have accepted the decision, despite expressing surprise that they had been criticised for their selection.
They said at the time that the 18-man match-day squad had a wealth of first-team experience although Mick McCarthy had made ten changes to his team from the one that had won at Tottenham.
Goalkeeper Marcus Hahnemann was the only player to keep his place in the starting line-up.
Chief executive Jez Moxey said of the ruling: "We're obviously disappointed but we respect the Premier League's ruling especially as they have now used our case as a clear warning to all other clubs, some of whom have made similar changes to their teams in Premier League matches in the past, that this is not acceptable in the future.
"We made a detailed representation to the Premier League and pointed to those precedents involving other clubs in previous seasons.
"This ruling may now lead to a wider discussion regarding the issue of squad rotation and the Premier League's rules.
"We look forward to fully participating in any such debate at future Premier League meetings."