Antonio Valencia's agent has confirmed Real Madrid have made a bid for the Wigan winger.
Wigan are resigned to losing the Ecuador international and Real Madrid and Manchester United are believed to be battling it out for his services.
Latics boss Steve Bruce has openly admitted the 23-year-old will move on in the summer as Wigan prepare to sell him to the highest bidder.
Real have held a long-term interest in Valencia and tried to sign him during the January transfer window, and they have now made the first moves to lure him to the Spanish capital this summer by tabling an offer.
United are also believed to have contacted Wigan to register their interest in the skilful wide-man with Sir Alex Ferguson a big admirer of the South American.
The player's agent Diego Herrera has admitted Real have bid for Valencia and that Wigan are awaiting other offers before allowing the player to leave.
"I believe Wigan will sell Antonio for the right offer and they will decide that once they receive that," Herrera told skysports.com.
"Real Madrid made an offer last week, but nobody from Wigan has told me of an offer from Manchester United.
"There are a number of interested clubs and I will speak with Wigan about it this week.
"Once the season is over I expect offers to be made and I will sit down with Antonio and decide what offer is in his best interests."