Wigan lost 2-0 to Romanian Champions League qualifiers FC Timiosara in their second friendly in Austria.
Both goals came in the opening 18 minutes as Roberto Martinez's side paid for lapses in concentration.
Arman Karamyan put the Romanians ahead in the 11th minute when he found space on the edge of the area to fire low past goalkeeper Chris Kirkland's left hand. Gigel Bucur added the second from close range after being left unmarked.
Martinez, who made nine substitutions at half-time, was not disheartened by the result despite it being their third friendly without a win.
"What interests me is how the players performed on the day and this was another fantastic training exercise against a team who were fitter than us and gave us a real challenge," he told the wiganlatics.co.uk.
"I think we took another big step forward and I was pleased with a lot of aspects of our game. Although I was disappointed with the way we conceded the goals they came from basic defensive errors and we can work on preventing those sort of mistakes happening again."