Juventus dropped points for the first time in 16 league matches as Bologna held the Serie A leaders to a goalless draw at the Stadio Renato Dall'Ara.
The Old Lady had won every league game since an October 28 loss to Sassuolo - and 13 straight in all competitions - but created little in their stalemate in northern Italy.
Manager Massimiliano Allegri left both 16-goal forward Paulo Dybala and winger Juan Cuadrado on the bench ahead of Tuesday's Champions League quarter-final clash with Bayern Munich and Juve carved out few openings in the pair's absence.
Patrice Evra twice came close in the opening exchanges while Paul Pogba lashed a couple of attempts wide and Cuadrado, on as a half-time substitute, almost reached a deflected cross at the back post late on.
But Juventus' run of 15 successive Serie A triumphs came to an end and they will now lose top spot on Monday if second-placed Napoli can defeat AC Milan too.
Ex-Manchester United full-back Evra found himself in advanced positions on a couple of occasions early on, heading wide from one corner before poking over as he stretched to meet Pogba's nod down.
At the other end Mattia Destro's long-range effort was pushed out by Gianluigi Buffon and Godfred Donsah smashed his first-time follow-up well wide when he had time to steady himself with a touch.
The visitors were posing little threat and it was no surprise to see Allegri turn to Chelsea loanee Cuadrado at the interval.
Juventus' next chance did come from down the right-hand side where Cuadrado was stationed, although it was from Stephan Lichtsteiner's cross that Pogba blasted a half-volley narrowly wide.
Dybala came on for the disappointing Simone Zaza with 22 minutes left and Juve found a sense of urgency in the closing moments.
Pogba picked up an inch-perfect pass from Claudio Marchisio in the area but lashed a left-footed try wide of the near post and Alvaro Morata's volley on the turn was kept out by home goalkeeper Antonio Mirante.
The stopper was needed again at the end to turn Pogba's cross between Morata and Cuadrado, who almost managed to flick the ball home as Juve's impressive run finally came to an end.
The result means that Napoli can return to the summit with a win on Monday night having only relinquished top spot following a loss to Juve in their most recent league fixture.
Source: PA
