Tranmere manager Les Parry is unlikely to have a trio of key players available for the trip to Bristol Rovers.
Ian Thomas-Moore, Ian Goodison and Adam McGurk are all major doubts after suffering knocks in Saturday's 3-1 loss to Colchester.
Thomas-Moore and McGurk only returned to the squad at the weekend following injuries but now look to be sidelined again with thigh and knee problems respectively.
Goodison did play a full part in the United game despite struggling with a neck injury, however he looks set to miss the game against the Pirates after picking up an ankle knock.
The size of Tranmere's casualty list is now a major concern for Parry with Gunnar Neilsen, Marlon Broomes, Ash Taylor, Liam Darville and Alan Mahon all still on the treatment table.