Tranmere boss Les Parry revealed defender Marlon Broomes and forward Enoch Showunmi are set to miss the trip to Notts County.
Broomes was withdrawn late in the second half of last weekend's home win over Plymouth after taking a blow to the hip/lower back area.
He is still troubled by the injury and will play no part against the Magpies.
Showumni is nursing a knee injury and will also miss out but veteran centre-back Ian Goodison should be passed fit despite swelling around his calf and shin.
Parry said: "Although Ian doesn't look like he's going to play at the moment, I expect him to play.
"He does pick up these injuries, he hasn't trained this week and I'll say he's 50-50 at the moment. But he'll play at the weekend and that's the way it works."