The Croatian had to be carried off on a stretcher after the former Arsenal midfielder flew in with a dangerous two-footed challenge on him in the second half of the 1-0 win in the San Siro.
The right-back left the stadium on crutches with a protective boot around the injured ankle that bore the brunt of the midfielder's reckless challenge.
Pain game: Vedran Corluka was carried off on a stretcher and could be out for a lengthy period
Corluka went for a scan on the ankle on Wednesday and will learn the results from a meeting with a specialist.
Tottenham manager Harry Redknapp lambasted Flamini for the tackle after the match, claiming that the 24-year-old was lucky not to break his leg.
The challenge sparked rash tackles and frayed tempers that resulted in Gennaro Gattuso head-butting Joe Jordan, but referee Stephane Lannoy adjudged that Flamini's tackle was only worthy of a yellow card.
Sidelined: Corluka was crocked by Matthieu Flamini's x-certificate challenge
UEFA announced that they had opened disciplinary proceedings against Gattuso, who could be hit with a lengthy ban.
Corluka's replacement for the last 35 minutes of the match, Jonathan Woodgate, suffered a groin injury during his time on the pitch and how long he will be out for should be known in the next 48 hours.
Spurs released a statement on Wednesday night saying that Woodgate had 'sustained a strain of the left adductor muscle.'
Another setback: Jonathan Woodgate's return came at a cost after picking up a groin strain
It was a cruel blow for the former Real Madrid defender, who was making his first appearance for the club after 15 months out with a long-standing groin injury.
The club are confident that he has not suffered a recurrence of his old groin injury and is expected to be out for weeks, rather than months.
I tried to sign mad-dog Gattuso last summer, reveals Tottenham chief Redknapp Woodgate injured on Spurs comeback in Champions League triumph in MilanMartin Samuel: Spineless UEFA will let off Flamini, the real San Siro villainAll the latest Tottenham news, features and opinion
Explore more:People: Jonathan Woodgate, Harry Redknapp