The Trinidad and Tobago international was hurt in a reserves run-out at Hetton on Tuesday night as he won a penalty to set his side on the way to a convincing 3-0 win over Wigan.
Jones, who has just finished a three-match suspension following his red card against West Ham in October, is believed to have suffered a dead leg.
Battle: Jones is giving Sunderland boss Bruce cause for concern
The striker tried to continue but indicated to the bench within a couple of minutes that he needed to be replaced.
Manager Steve Bruce, who appeared anxious as he looked on from the stands, had wanted to use the game to give Jones a fitness boost ahead of Sunday's trip to Fulham and he will now be monitored over the coming days.
[item name=module id=1017989 style=4 /]Bruce said: 'Kenwyne's got a dead leg and we expect him to be OK for the weekend. To lose him again would be a blow, but hopefully things will be fine and he has an extra day to recover with the game not being until Sunday.'
Teenager Ryan Noble, who was promoted to train with the first team last week, scored a hat-trick, including the opener from the spot as Wigan were swept aside.
The Sunderland-born striker has now notched 27 goals for the reserves and Under 18 teams this season.
But there was a blow for Scotland defender Russell Anderson who was sent off for a professional foul in injury-time in his first 90-minute run-out for more than a year.
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