Sam Allardyce and his Sunderland counterpart Ricky Sbragia rested their big guns for the initial clash but were punished by having to add another fixture to their already busy schedule.
The tie will be the fifth time the two sides have met in all competitions this season with both sides recording one win apiece.
The winner of the clash faces a home match against Championship club Coventry in the fifth round.
Rovers' team newsAllardyce will look to give some of his more regular starters a rest on Wednesday with midfielder David Dunn and striker Benni McCarthy most likely to benefit.
This could give striker Roque Santa Cruz the opportunity to partner Jason Roberts up front.
Midfielder Brett Emerton has been ruled out for the rest of the season with cruciate knee ligament damage.
On-loan Manchester United defender Danny Simpson could see himself called upon to fill the void left by the Australian.
Sunderland team newsGoalkeeper Craig Gordon is set to start his first game in ten weeks after recovering from a fractured ankle suffered in training.
The Scotland international has not played since the embarrassing 4-1 home defeat at the hands of Bolton and knows he has a real fight on his hands to reclaim the No.1 jersey from Marton Fulop.
Sbragia has admitted that some of the club's promising youngsters may get their chance for the cup clash with full-back Michael Kay set to start and reserve team captain Jack Corback also in contention.
Preferred strike pairing of Djibril Cisse and Kenwyne Jones are set to be given the night off after both picked up minor knocks in the Tyne-Wear derby on Sunday.
New arrivals Tal Ben Haim and Calum Davenport are both ineligible to play while fellow defenders Nyron Nosworthy (hamstring), Anton Ferdinand (minor injury) and Paul McShane (cup-tied) are all unavailable.
Midfielder Teemu Tainio joins them on the sidelines with a minor knock.