Stoke manager Tony Pulis accused Arsene Wenger of being "out of order" following the Arsenal manager's criticism of defender Ryan Shawcross.
Pulis revealed that Stoke have written an official letter of complaint to the Gunners and also sent copies to the Premier League and the Football Association.
The row surrounds Wenger's comments earlier this week accusing the Stoke centre-back of turning football into rugby. The Arsenal boss was fuming with Shawcross in February after his horror tackle left Aaron Ramsey with a sickening leg break - an injury the Wales midfielder is still recovering from.
His latest attack on Shawcross came after he was incensed by what he claimed were more rough tactics from the defender during Stoke's 2-1 defeat at home to Tottenham last Saturday.
Wenger commented: "You cannot say it is football any more. It is more rugby on the goalkeepers than football. When you see the way Shawcross kicked Heurelho Gomes, how Robert Huth pushed Gomes in the goal, you cannot say that is football anymore."
Pulis responded: "The club is desperately disappointed with the comments that Wenger has come out with, especially the comments about Shawcross. We think they are out of order and you don't expect it from a person of Wenger's esteem. The club will deal with it in the right and proper manner."