Sheffield United manager Micky Adams hopes Nick Montgomery, Richard Cresswell and Ched Evans will all be back in contention against Cardiff.
Midfielder Montgomery's wife recently gave birth to twins and strikers Cresswell and Evans also missed Saturday's home defeat to Middlesbrough due to respective groin/Achilles and knee injuries.
Adams will again be without midfielder Lee Williamson, who sits out the last game of a four-match ban.
Teenage midfielder Erik Tonne, signed from Strondheim in the January transfer window, made his first appearance as a substitute on Saturday and could feature again while striker Marcus Bent and winger Mark Yeates, who also stepped off the bench on Saturday, are pushing for recalls to the starting line-up.
The defeat to Middlesbrough saw them slip to the bottom of the table for the first time this season and they remain seven points from safety with six games left.