Scunthorpe's new signings Jamie Reckford, David Mirfin, Jon Parkin and Robbie Gibbons are all expected to be included in the squad for the home game with Walsall.
Teenager Reckford, signed on a month's loan from Wolves, and fellow defender David Mirfin, returning to the club where he spent three years from Watford until the end of the season, will boost manager Alan Knill's back four.
Striker Parkin has signed on loan for a month from Cardiff, while midfielder Gibbons is a permanent free signing following his release from Cypriot side Alki Larnaca.
Defender Christian Ribeiro (concussion) is expected to be included after being involved in an aerial collision against Sheffield Wednesday last week, and skipper Cliff Byrne (groin) and midfielder Damien Mozika are close to making their returns.
Byrne, dogged by injury for over a year, has recently been struggling with a groin injury, while summer signing Mozika has missed the last three-and-a-half months due to an ankle problem.
Midfielders Jimmy Ryan (knee) and Michael Collins (Achilles), plus strikers Connor Jennings (foot) and Matt Godden (hip) remain sidelined.
Walsall are set to be without experienced duo Jon Macken and Jimmy Walker.
Veteran striker Macken was forced off with a hamstring injury after just 37 minutes of Tuesday's 1-0 home defeat to Notts County while goalkeeper Walker was substituted at half-time nursing a similar problem.
Manager Dean Smith told the club's official website: "Having assessed both players we don't think they are as bad as first feared. They are both going for a scan which will tell us exactly how bad it is and then we'll be able to make a judgement on how long they will be out for.
"Obviously we don't want to be without them for too long but it gives an opportunity for other players to come in and stake their claims.
It's important the likes of David Grof, George Bowerman, Will Grigg and Ryan Jarvis are ready to step up to the plate."
Smith has no other injury or suspension concerns going into the important clash, with the Saddlers just one place above Scunthorpe - who occupy the final relegation berth - by virtue of goal difference.
Scunthorpe V Walsall - view commentary, squad, and statictics of the game live.
Source: PA
