John Breckin will take charge of the team on Saturday and he will certainly be without striker Tom Pope.
The summer signing from Crewe is sidelined with an ankle injury, but the damage is not as serious as the Millers had feared and he is expected to be out for two to three weeks.
Pablo Mills is a doubt after picking up a groin injury in the Johnstone's Paint Trophy defeat to Huddersfield on Tuesday night.
Striker Paul Warne picked up a calf injury in the warm-up in midweek but could return against the Spireites and Michael Cummins hopes to be passed fit despite injuring his shoulder against the Terriers.
Veteran striker Drewe Broughton (broken toe) came off the bench against Lee Clark's side and will be in contention once again, but Nick Fenton (groin) is definitely out.