Real Madrid have failed with their appeal to UEFA to be allowed to register recent signings Klaas-Jan Huntelaar and Lassana Diarra for this season's Champions League.
The Spanish giants had hoped to win special dispensation to register the duo, despite them having played for their previous clubs in this season's UEFA Cup.
Current regulations allow either Huntelaar or Diarra to feature to Real this term, with UEFA standing behind their current regulations.
A UEFA statement read: "UEFA's appeals body upheld the decision taken by the UEFA control and disciplinary body on January 8, in accordance with article 17.18 of the UEFA Champions League competition regulations.
"They rejected an appeal by Real Madrid in relation to the Spanish club's request to be able to register two players who have already competed in UEFA competition this season, as part of their A-list squad for the knockout rounds of the UEFA Champions League."
Dutch striker Huntelaar featured in European competition for Ajax earlier this season, while France midfielder Diarra played for Portsmouth in the UEFA Cup.