Portugal suffered a major injury blow on Tuesday after it was announced winger Nani would play no part in the World Cup after damaging his collar bone.
The Manchester United star is thought to have suffered the injury in training on Friday and will be replaced in the squad by Benfica midfielder Ruben Amorim.
A statement on the Portuguese Football Federation's official website, www.fpt.pt, read: "Following the injury to the left clavicle suffered by Nani, a daily assessment of the damage and the completion of medical tests, we have concluded he is unable to be competitive."
"The medical file will now be presented to the medical department of Manchester United.
"In light of the report, the Portuguese Football Federation, taking into account the FIFA regulations for the 2010 World Cup, will call-up Ruben Amorim as a replacement."