Portugal defender Fabio Coentrao has been ruled out of the remainder of the World Cup with a thigh injury.
The Real Madrid full-back pulled up sharply during the second half of Portugal's 4-0 defeat to Germany on Monday night and was replaced by Andre Almeida.
Coentrao underwent scans on the injury on Tuesday and the Portuguese football federation confirmed afterwards that the 26-year-old would play no further part in the tournament.
An FPF statement read: "According to the tests, Fabio Coentrao is unfit for competition for a period exceeding the duration of the World Cup."
Striker Hugo Almeida (hamstring) and goalkeeper Rui Patricio (thigh) also underwent scans on injuries picked up in the match but neither have been ruled out of the tournament yet.
Portugal's heavy defeat to Germany, which saw Pepe sent off in the first half, has left them bottom of Group G with matches against the USA and Ghana to come.