Yeovil claimed West County bragging rights over neighbours Plymouth with a 1-0 victory at Huish Park.
The two struggling sides battled out a goalless draw a month ago and once again found goals hard to come by.
Argyle had the better of the first half but impressive goalkeeping from Stephen Henderson kept out the dangerous Rory Fallon. Yeovil returned everything thrown their way and grabbed the vital breakthrough after 66 minutes.
A right-wing corner from Andy Walsh was partially cleared back to him and his second attempt was knocked in from 12 yards by Andy Williams.
A wonderful save from Henderson then denied Stephan Zubar and kept Yeovil in front.
And then deep into stoppage time Argyle's Yannick Bolasie was sent off for a bad foul on Henderson.