Ally Gilchrist and Scott Ross out for Peterhead
Peterhead can clinch second spot by defeating visitors Ayr but they will be without Ally Gilchrist and Scott Ross who have groin injuries.
Ryan Strachan and Rory McAllister will return after missing the 5-1 defeat by Brechin City last weekend with manager Jim McInally giving Simon Ferry close to kick-off time to prove his fitness after a knee injury
McInally said: "We were short of defenders at Brechin and it showed. We will be looking to do a lot better as defeating a good Ayr side would give Peterhead their highest ever league finish.
"That would be a fine achievement before we start the play-offs."
The Somerset Park side miss Brian Gilmour who has an ankle injury with Jamie Adams also absent due to a knee problem.
Ryan Stevenson and Alan Trouten are both carrying knocks which may see manager Ian McCall have to leave them out.
McCall said: "We had a good 4-0 win at Stenhousemuir last weekend with a display that was our best for a few weeks. We want to build on that against a Peterhead side that will have been hurt by losing so heavily last weekend.
"It will be great to win and go second but this game will not have any relevance for when we meet in the play-offs."