Midfield man Danny Whitaker missed Tuesday's 2-0 defeat at Millwall because he was unwell.
Whitaker had been named in Penney's 18-man squad but was unable to travel after being struck down by a bug.
He will be assessed but summer signings Rob Purdie and Joe Jacobson remain sidelined due to injury.
Midfielder Purdie is recovering from a stomach injury and defender Jacobson is currently troubled by a hernia.
Striker Lewis Alessandra has joined Chester on a month's loan and could go straight into their starting line-up for Saturday's daunting trip to Luton.