Ameobi, who scored a hat-trick against the Royals in Newcastle's opening home game of the season, makes his first start for three matches at the Madejski Stadium.
Chris Hughton has made three changes to the side that beat Blackpool 4-1 at the weekend, with full-back Ryan Taylor and midfielder Alan Smith also returning to the starting line-up.
Tamas Kadar, Peter Lovenkrands and Andy Carroll are the players to make way, with Jose Enrique named amongst the substitutes after recovering from injury.
READING (4-4-2): Federici; Griffin, Gunnarsson, Pearce, Bertrand; Kebe, Tabb, Howard, McAnuff; Long, Sigurdsson.
Subs: Hamer (gk), Cisse, Henry, Church, Robson-Kanu, Karacan, Hamer, Rasiak.
NEWCASTLE (4-4-1-1): Harper; R Taylor, Coloccini, Williamson, Simpson; Routledge, Smith, Guthrie, Gutierrez; Nolan; Ameobi.
Subs: Krul (gk), Enrique, Barton, Pancrate, Lovenkrands, Carroll, Best.