The Magpies suffered their second consecutive defeat at the hands of Scunthorpe in a gritty encounter at Glanford Park.
Martyn Woolford's brace made certain of three points for the home side, whilst condemning Hughton's team to another league defeat.
Hughton is due to meet Mike Ashley at the weekend to discuss a permanent role at St James' Park and the 50-year-old is still confident regarding talks for the manager's post.
"It will have no effect at all," he said of the defeat.
"As far as I know they (the talks) are still going ahead. I'm still very confident the situation hasn't changed."
Game planKevin Nolan's equaliser midway through the second half had brought the Magpies level at one stage, but they were unable to fend off the challenge of their hosts.
"The game plan was to come here and do well," he said.
"On the balance of play and chances it's a game we should have won, but we have to do better.
"They were able to turn us at times, they were always a threat and had a lot of energy in the side and at the clinical moments they got the goals.
"We didn't defend moments well enough and that enabled them to get the goals that they did. On the balance of play it's a game that we should have won.
"I think this is a pressure on us. We are a side in this division that everybody expects to do well and certainly our own supporters expect us to do well and that's the pressure that we carry with us in every game. That's a pressure that we've got to live with."
DelightedScunthorpe manager Nigel Adkins expressed his delight in securing all three points in front of a record crowd.
"It's quite simple, we haven't hidden away from the fact that we have the smallest fan base and the smallest budget in the division," Adkins said.
"But it's what you do on the field of play. We've got a fantastic team spirit.
"This is a great victory. When we got promoted, this was the first game everybody was looking for; Newcastle United at home.
"We packed Glanford Park out, played against a top side and we have gone and got a victory."