The Australian is preferred to regular number one Danny Coyne as he ends a 15-match exile.
Jones is one of five changes to the side that scored a late equaliser to claim a point at Derby on Tuesday.
Both full-backs change, with Justin Hoyte lining up at right-back instead of Kyle Naughton and Jonathan Grounds preferred to Andrew Taylor in the left-back berth.
Emanuel Pogatetz replaces the injured Stephen McManus at centre-half, while Jonathan Franks deputises for Rhys Williams in midfield. Williams is suspended after being sent off in the closing stages of Tuesday's draw.
MIDDLESBROUGH (4-4-2): Jones; Hoyte, Wheater, Pogatetz, Grounds; Franks, O'Neil, Robson, Arca; Killen, McDonald.
Subs: Coyne (gk), Taylor, Riggott, Lita, Emnes, Miller, Naughton.
READING (4-4-2): Federici; Griffin, Mills, Ingimarsson, Bertrand; Kebe, Tabb, Howard, McAnuff; Long, Sigurdsson.
Subs: Harper (gk), Gunnarsson, Matejovsky, Church, Robson-Kanu, Rasiak, Khizanishvili.