Boss Sir Alex Ferguson said: "Rio has been training very well in the last few weeks. He will play on Saturday."
Ferdinand has featured only eight times for his club this season.
He has missed 19 games for United, and also sat out England's friendly with Brazil, after suffering what was reported to be a calf injury believed to be related to a long-standing back problem.
England boss Fabio Capello will also be pleased by Ferdinand's return, with the Italian having already made it clear that he will take only fully fit players to this summer's World Cup in South Africa.
Hopefully seeing Rio, Vidic, Evra, Brown and VDS back in defence can give us more of a will to attack Into Sheringham!, AND SOLSKJAER HAS WON IT Ferdinand underwent intensive treatment from a specialist in London and Ferguson is now happy that his player is ready to play again.
"This is the time to bring him back," added the Scot. "We have gone through the whole situation with him in terms of enough training and endurance work.
"It is a big boost because the defence has been the weakest part of our game this season.
"At various times we have lost Ferdinand, Nemanja Vidic, Edwin van der Sar, John O'Shea.
"It has been a rough time, so to get them back can only be good for us."
Ferguson confirmed Vidic was still a fortnight away from recovering from a calf complaint, although that would at least put him in contention to face AC Milan in the Champions League knock-out round.
He revealed this week that O'Shea could miss the remainder of the season after suffering a blood clot in his leg, but Ferguson said Dimitar Berbatov will be available on Saturday after recovering from a dead leg.
Meanwhile, Ferguson has revealed he will select Owen Hargreaves in his 25-man Champions League squad even though he has no real idea when the midfielder will make his comeback.
Hargreaves has been missing for over 15 months after undergoing major surgery on both knees in an effort to cure a long-standing tendinitis problem.
"Owen is still training with the reserves," said Ferguson. "Now it is two days on and one day off and he is preparing to get to the proper levels.
"He has done two or three sessions with us in the last couple of weeks and did very well in Qatar last week.
"But it is a process and hopefully we will get to the point where he feels confident about playing again."