England defender Rio Ferdinand has been ruled out Wednesday's World Cup qualifier with Andorra through injury.
The Manchester United centre-back missed Saturday's 4-0 win in Kazakhstan due to discomfort with a calf injury.
It was expected Ferdinand would join up with his England colleagues on Sunday but it has now been confirmed the 30-year-old will miss the game at Wembley.
Midfielder Gareth Barry will be absent through suspension after picking up his second booking of the campaign.
In Ferdinand's absence, Matthew Upson partnered John Terry in the centre of the England defence during Saturday's win in Kazakhstan; a sixth successive qualifying Group Six victory under manager Fabio Capello.
There was little prospect of England taking any chances with Ferdinand against Andorra - a team ranked 196th in the world and without a single point yet in this World Cup qualifying campaign - even though a victory would leave Capello's side on the brink of World Cup qualification.
Ferdinand's exclusion ends an injury-blighted campaign for the player.
He was troubled by the calf injury in the build-up to the Champions League final - but played in Manchester United's 2-0 defeat by Barcelona in Rome - and has also been troubled by a back complaint in recent times.
Bolton's Gary Cahill was called up to squad by manager Fabio Capello last week for the first time as Ferdinand's replacement.
Capello has opted not to call up a replacement for midfielder Gareth Barry, who scored England's first goal in Almaty, but is suspended for Wednesday's match at Wembley after being booked in the first half.
England struggled at the start in Almaty but ended up winning comfortably thanks to goals from recent £12m Manchester City signing Barry, Emile Heskey, Wayne Rooney
and Frank Lampard.