Captain Craig Bellamy is out of Wales' Euro 2012 qualifiers against Bulgaria and Switzerland next month.
Bellamy, currently on-loan at Cardiff from Barclays Premier Leagueside Manchester City, has been sidelined for three weeks with a kneeproblem that has hampered him throughout his career.
Bryan Flynn, caretaker boss of the national side, confirmed that Bellamy would miss the forthcoming qualifiers.
'Craig will be unavailable for the two matches next week,' Flynn told BBC Sport Wales.
'I had a really long, excellent conversation with Cardiff managerDave Jones just over 10 days ago.and we made the decision that Craigwill not be available due to his fitness.
'He has an ongoing knee problem and hasn't played for the last five games.
'Obviously we've been aware of that and unfortunately for Craig andfor us he won't be able to put on a red shirt next week. It's a bigdisappointment for me as caretaker manager. I wanted Craig in the teambut these things happen.'
Wales lost their opening Group G game against Montenegro and faceBulgaria at the Cardiff City Stadium on October 8, followed bySwitzerland in Basle four days later.
The defeat to Montenegro saw John Toshack depart as manager andWales Under-21 coach Flynn appointed on a caretaker basis in his place.
Meanwhile, former Wales striker John Hartson today confirmed he had applied for the manager's job.
Give me the job: Former Wales striker John Hartson
'I applied for the Wales job,' Hartson said.
'I'm 35 now and have got all my (coaching) badges. They gave Mark Hughes his first job at 35 with no experience behind him.
'I know the players, I know the system I would play, although that could change from game to game.
'I feel as if my time has come. I've been retired for three years.Brian Flynn is a great guy, I worked with him for the under-21s.
'He is a brilliant coach and if I was to miss out to anyone, Brian is a brilliant guy. I wish him well for the next two games.
'If I get it, I will be the happiest man in Wales and if I don't, I move on.'
Bellamy 'coin attack' in Manchester derby thrown out of court'I had to go': Toshack resigns as Wales manager after six yearsMontenegro 1 Wales 0: Toshack and Collins floored in Montenegro