Macclesfield manager Gary Simpson felt his side were undone by playing one game too many after watching a clinical Cheltenham outfit claim a 2-0 win.
The home side had their chances in a strong first 45 minutes but it was the visiting Robins who went home with the points after goals from Josh Low and Brian Smikle.
Simpson said: "We had a number of chances, and by that I mean really good chances, to score in the first half but we couldn't put one away.
"After the form they've shown in recent weeks you can't have a go at the players but we did need one of those to lift us. I felt that we were playing on empty. It was really one game too far for us and we looked very leggy.
"Cheltenham are a quality side with a lot of pace and in the second half they sat back, soaked up our pressure and looked very dangerous on the break and to be fair they could have had even more goals.
"I'm disappointed at the result but we've got a young group of players and we've just to pick ourselves up and go again."