The Spanish defender played in Liverpool's first two games of the season after Martin Skrtel and Daniel Agger were struck down by injuries.
Rafa Benitez then ventured into the transfer market to sign experienced Greek international Sotiris Kyrgiakos and Ayala realises he will now have to wait for his next chance.
But he is determined to prove his worth in training and put as much pressure on the senior centre-backs as possible.
"I need to keep working hard and prove myself," said Ayala.
"If I keep training well and playing well for the reserve team then maybe I might get another chance in the Carling Cup later this month."
Fantastic experienceAyala came on as a substitute in the opening day defeat at Tottenham before making his full debut in the 4-0 win over Stoke.
He said: "It was a fantastic experience to play in front of such a big crowd against Tottenham and I was very excited.
"The manager just told me to go out and play my normal game and enjoy it.
"Then he told me on the Tuesday that I was going to start the next night against Stoke. I was more nervous than I was at Tottenham because it was to be my first senior start at Anfield.
"I was excited to be playing in front of the Kop and when we came out the noise was incredible.
"It was a very special experience to play my first full first team game at Anfield, and to keep a clean sheet made me very proud.
"The manager told me well done at the end of the game, and to keep working hard."