Leyton Orient will be without their central-defensive pairing Ben Chorley and Terrell Forbes for the trip to Tranmere.
The duo are suspended after they were sent off during Tuesday night's extraordinary 8-2 FA Cup replay win over non-league Droylsden.
Chorley sits out a one-match ban after picking up two yellow cards while Forbes will miss three games for a straight red.
Aaron Brown will fill one of the gaps at the back while Brighton loanee Andrew Whing, who was ineligible to play in the cup, could come in at right-back with Elliot Omozusi moving across to the centre.
Midfielder Adam Chambers is still recovering from surgery on his calves and goalkeeper Jamie Jones is out after a hernia op.