Arturo Vidal and Giorgio Chiellini struck in either half to hand Juventus a 2-0 away win at Bologna on Friday which gave the Serie A champions a six-point lead over Roma.
Four days ahead of a crucial trip to Istanbul, where Juve need a point against Galatasaray to qualify for the last 16 of the Champions League, coach Antonio Conte left a host of regulars on the bench.
Fabio Quagliarella and Mirko Vucinic led Juve's attack leaving Carlos Tevez and Fernando Llorente to make later appearances, while Angelo Ogbonna replaced centre-back Leonardo Bonucci.
Mauricio Isla and Federico Peluso were also deployed as wing backs, in place of Stephan Lichsteiner and Kwadwo Asamoah, while Paul Pogba took injured Andrea Pirlo's role at the centre of the five-man midfield.
Despite the reshuffling, Juve had no trouble dictating play against a side that has only one home win so far this season and were sent crashing out of the Italian Cup by Serie B side Siena, in Bologna, on Tuesday.
Juve should have had several goals by the interval but Quagliarella and Claudio Marchisio spurned early chances against impressive home 'keeper Gianluca Curci.
The visitors eventually found the net when Peluso's cross found Vidal unmarked and the Chilean had no trouble sending it over the onrushing Curci.
Quagliarella then sent an effort go just wide under a challenge from Mikael Antonsson while at the other end Gianluigi Buffon produced two solid blocks to deny long-range efforts from Alessandro Diamanti, the first a free kick.
Two minutes later Bologna saw claims for a penalty waved away after the ball played Vucinic's arm.
The Montenegrin had earlier came down hard under a challenge and was replaced by Llorente just before the break.
Bologna threatened briefly at the end of the half, when Vidal saw a shot go wide after Llorente's dribbling run left the Chilean with a clear shooting chance.
Curci really came into his own as Bologna suffered in the second half, the Italian blocking at Llorente's feet after he had been set up by Ogbonna and saving again two minutes later after Llorente spun on Peluso's short pass to the right of goal.
Conte replaced Peluso with Asamoah and a minute later the Ghanaian had the ball in the net, only to be ruled offside.
Tevez replaced Quagliarella shortly after and although a constant threat Juve survived a scare when late substitute Jonathan Cristaldo pounced on Chiellini's poor clearance to fly past Ogbonna and send an angled drive just past the upright.
Bologna threatened again when Archimede Morleo got on the end of Diamanti's pacy cross from the right to send a bouncing header a foot wide of Buffon's post.
On 85 minutes, Curci did well to deny Llorenta again when he parried the striker's shot from close range.
But the 'keeper could do little when Chiellieni got his head to a corner at the near post to send Curci sprawling into the net in the 89th minute.
Roma, second at six points, are in action on Sunday but their potentially tricky clash with Fiorentina comes on the back of four consecutive draws.