Bullard, who has played just 37 minutes of first-team football since his £5million move from Fulham in January, struck a long-range opener in the second string's 2-1 home loss to Bolton.
The 30-year-old, recovering from a knee operation, received the ball on the left and fired into the top corner from the edge of the area on the stroke of half-time at rain-soaked North Ferriby.
It proved his last touch of the ball as he was substituted at the interval.
Bullard injured his knee on his Hull debut at West Ham in January and consequently underwent major surgery on the joint for the second time in less than two-and-a-half years.
Reserve-team boss Aidan Davison said: "It was the next step in his rehab, 45 minutes from him was plenty. There is absolutely no problem at all. It was business as usual for him."