Colin Calderwood's miserable start as Hibernian manager continued when a brilliant piece of forward play from Ayr veteran Mark Roberts sent the Edinburgh side crashing out of the Scottish Cup with a 1-0 defeat.
The 35-year-old's impressive footwork and perfect finish in the 19th minute decided the fourth-round replay and left Hibernian fans with little to look forward to for the rest of the campaign other than retaining their Clydesdale Bank Premier League status.
Hibs found Ayr goalkeeper David Crawford in excellent shot-stopping form but they struggled to consistently create chances and are now without victory in nine games.
The home players appeared to find extra energy as they chased every ball before inevitably being pegged back in the final 10 minutes.
But Crawford held Derek Riordan's powerful strike and the home defence charged down every other effort on goal before the final whistle sparked a pitch invasion from the jubilant home fans.