And Jackson feels the Hibs boss will demand atonement from his players after taking one Scottish Premier League point from their previous two home games.
"Yogi will not let that happen," said Jackson when asked if Hibs could underestimate the Ayrshire side.
"Especially because they have had quite a heavy defeat by Rangers and a draw against Hearts in two home games.
"And you saw what happened at Old Trafford on Sunday, that will remind people that if Leeds can go there and win, anything can happen."
Hibs are still comfortably in third place in the SPL despite dropping points in recent weeks and Jackson feels Hughes is doing "exceptionally well" in his first season at the helm.
"Everyone knows his philosophy, that he wants to play football, but his desire, enthusiasm and commitment just has to get through to the players," the former Scotland forward said.
"Even before the game he's walking up and down the touchline when they're warming up, he is talking to fans and having a laugh with them, and going on the pitch and playing keep-ball with the subs.
"His enthusiasm is incredible and his man-management is fantastic as well because he seems to get players who have kind of come off the rails a wee bit, put an arm round them and make them believe in themselves.
"When you are getting that passion from the manager, you are up for it and want to get out there, and when you are winning games you love it too."
Hibernian 1/10, Draw 9/1, Irvine Meadow 18/1