Fiorentina sacked coach Delio Rossi after he physically attacked midfielder Adem Ljajic during Wednesday night's 2-2 draw with Novara.
The attack came after the player's apparent dissent at being substituted just 32 minutes into the match.
Fiorentina president Andrea Della Valle said in a statement on the club's official website: "In a few seconds months of stress came out. I am sorry because the coach has never committed acts of this kind. For his own good to make him understand he has made a mistake I had to take this decision. No provocation justifies this reaction."
Della Valle confirmed Ljajic would also face action for his part in the incident, and said he would wait to announce who would take charge of the side for the rest of the season.
"Also for respect of the person with whom I believed in to reconstruct Fiorentina I will say nothing official until tomorrow," he added.
"Then you will know who will be given the reins of the team. We will also take proportionate measures against Ljajic.
"Rossi has not justified it to me but he is someone who has never done anything like this.
"I am sorry also that in the 10th year of my reign and with what he has brought - we have won at Milan and Roma and I believed this evening we would have achieved safety, certainly not that I would have to take a decision like this."
The dramatic events unfolded as the Viola fought to confirm their the Serie A status. The point was enough to put them six points clear of the drop zone with two games remaining.
Television pictures clearly show the coach attacking Ljajic as he took his place on the bench. The player appeared to sarcastically clap his coach's decision to substitute him, and Rossi responded by throwing punches as others on the bench attempted to restrain him.