Daniel Sturridge was unable to complete a full training session with England, raising doubts about his participation in Tuesday's game against Germany.
Sturridge trained with his England team-mates for 45 minutes on Monday morning before he left the training field with physio Steve Kemp.
The Liverpool striker has been carrying a thigh injury but said on Sunday that he was happy to play against the Germans.
Sturridge was not hobbling when he walked off the pitch, but the fact that he did not complete the session will be a concern for manager Roy Hodgson the day before the clash with Joachim Low's men at Wembley.
The good news for Hodgson is that captain Steven Gerrard, striker Rickie Lambert and defender Kyle Walker all trained despite missing the defeat to Chile last week through injury.
Jack Wilshere was also present despite suffering a dead calf at Wembley last Friday.
Joe Hart, who has been on the bench for the last four Manchester City games, looked keen to impress, taking to the field 30 minutes early along with fellow goalkeepers Fraser Forster and John Ruddy.
After 30 minutes of aerobic work, Hodgson's squad split in to two teams for a practice match.
The line-ups gave a hint as to who may start the Friendly against Germany.
Walker, Chris Smalling, Phil Jagielka and Ashley Cole lined up in front of Hart in what looked to be the first-choice XI.
The midfield consisted of Gerrard, Tom Cleverley, Adam Lallana and Andros Townsend. Wayne Rooney sat just behind Sturridge up front.
Everton midfielder Ross Barkley replaced Sturridge when he went off, with Rooney pushing forward into the main striker's position.
Hodgson is expected to give an update on the fitness of his players at a 1.30pm press conference.