Liverpool's Joe Gomez has been recalled to the England Under-21 squad after 18 months out ahead of their double header against Germany and Denmark.
The 19-year-old defender had been sidelined since suffering a serious knee injury in the Young Lions' Euro 2017 3-0 qualifying win over Kazakhstan in October 2015.
He returned for Liverpool in January and has made three appearances since regaining fitness.
Southampton midfielder Sam McQueen is also primed for his debut after a first call-up while regular Young Lions captain and Saints team-mate James Ward-Prowse has been selected for the senior squad for the first time along with St Mary's colleague Nathan Redmond.
Aidy Boothroyd's side travel to Wiesbaden to face Germany on March 24 before a game against Denmark in Randers on March 27 as they begin their preparations for Euro 2017 in Poland this summer.
Boothroyd, who replaced senior manager Gareth Southgate on a permanent basis in February, told the FA's official site: " These are two very testing games.
"Although I've been in the job on an interim basis, for my first game to be Germany away - they don't come much more difficult than that.
"But that's what we want. We want to test ourselves against the best."
Tottenham's Harry Winks, Chelsea striker Tammy Abraham, who has scored 21 goals on loan at Bristol City this season, are in along with Leicester's Demarai Gray ahead of the friendlies.
Squad: Gunn (Manchester City), Pickford (Sunderland), Walton (Brighton), Chilwell (Leicester), Gomez (Liverpool), Hause (Wolves), Holding (Arsenal), Holgate (Everton), Mawson (Swansea), McQueen (Southampton), Stephens (Southampton), Baker (Vitesse Arnhem - on loan from Chelsea), Chalobah (Chelsea), Grealish (Aston Villa), Hughes (Derby), Loftus-Cheek (Chelsea), Swift (Reading), Winks (Tottenham), Abraham (Bristol City - on loan from Chelsea), Brown (Huddersfield - on loan from Chelsea), Gray (Leicester), March (Brighton), Woodrow (Burton - on loan from Fulham).