Gary Lewin says he is 'absolutely devastated' to be going home after breaking his ankle in a freak accident during England's 2-1 defeat to Italy.
Lewin, attending his 205th match as England physio, fell over in the technical area on Saturday night as he celebrated Daniel Sturridge's equaliser in Manaus.
Scans revealed he had dislocated and fractured his ankle in what manager Roy Hodgson described as a "very painful" accident.
Lewin flew back to Rio from Manaus with the team but he will now return to the UK. England's other physio Steve Kemp will take on Lewin's duties while Hodgson's men remain in the tournament.
Lewin said: "I'm absolutely devastated to be going home.
"A lot of hard work, planning and preparation has gone into this tournament.
"England has a fantastic backroom staff who will continue to provide excellent support to the players and management.
"I'd like to thank my England colleagues and the FIFA medical officers at the Amazonia Arena for their excellent, professional treatment.
"And of course, I wish the England team all the best for the rest of the tournament."
Hodgson said he was sad to hear Lewin, England physio since 1996, had been ruled out of the tournament.
"It's very unfortunate for Gary and us that this accident occurred," the England manager said.
"We will of course miss him as he's an extremely experienced and popular member of staff.
"However, we wish him a speedy recovery and know he'll be cheering us on from back home."
Lewin is the second England staff member to suffer an injury on this tour.
Assistant coach Ray Lewington needed surgery on his knee after waking up in agony during the squad's warm-up camp in Miami.
Lewington has remained with the squad, although he has been given a cast for his left leg.