Gabriel Agbonlahor and Micah Richards have both pulled out of the England Under-21 squad to face Norway and France due to injury.
Aston Villa striker Agbonlahor and Manchester City defender Richards both have hamstring strains.
Both players were added to the squad at the beginning of the week despite carrying knocks from games over the weekend.
Under-21 coach Stuart Pearce has decided not to call up a replacement for either.
"Both players arrived with injuries and have had treatment with the medical staff, but they have not responded to that treatment," said a spokesperson for the Football Association.
"They have now been sent back to their respective clubs as a precautionary measure."