Michael Carrick missed training on Monday as England prepare for their must-win World Cup encounter against Slovenia.
The Manchester United midfielder sat out the session at the Royal Bafokeng Sports Campus after taking a blow to the ankle during training on Sunday.
He joined groin injury victim Ledley King of Tottenham on the sidelines ahead of the make-or-break game in Port Elizabeth on Wednesday.
The Three Lions must win against Slovenia to qualify for the last 16 following lacklustre draws against the United States and Algeria during a much-criticised campaign to date.
Meanwhile, Slovenia coach Matjaz Kek has warned England that his players believe they can win the pivotal game to seal their own passage to the knockout stages as group winners.
"The most important thing for such a small country as Slovenia is belief," he said following an opening 1-0 victory over Algeria and 2-2 draw with the United States on Friday.
"You must believe you can achieve, then you have a chance. That goes for any team and my team believe they can beat anyone.
"I'm not thinking 'okay, we came to South Africa but we're small and we must try to avoid catastrophe'.
"It is more 'can I win against every team?' That is my objective."