England captain David Beckham missed training on Monday after picking up a minor ankle injury during Saturday's 6-0 win against Jamaica.
Ashley Cole and John Terry, who were substituted during the first half of the rout after suffering thigh and knee injuries respectively, joined Beckham on the sidelines.
However, none of the problems are serious and the trio were only rested as a precaution.
There was some good news during the final training session before England departed to their World Cup base in Germany, as injury doubts Gary Neville and Wayne Rooney were both able to undertake some light work.
Football Association spokesman Adrian Bevington said: "Gary Neville and Wayne Rooney were working with the physios during the session.
"Gary is expected to train tomorrow while Wayne will continue with the rehabilitation of his foot with England's medical team.
"John Terry, Ashley Cole and David Beckham, who had a kick on his right ankle on Saturday, did not train.
"This was purely precautionary with all three players. Sven fully expects all three to be training this week and available for Saturday's opener against Paraguay."