West Ham United striker Dean Ashton will not make his England debut against Greece on Wednesday after suffering an ankle injury in training.
New England head coach Steve McClaren had pencilled in Ashton to lead the line at Old Trafford, but the 22-year-old is instead likely to spend the next few days wondering whether he will be fit for the Hammers' Premiership opener against Charlton.
McClaren said: "It's disappointing when a young player is given his chance and gets injured.
"Dean is having a scan at the hospital and we're awaiting the outcome."
The injury to Ashton means Peter Crouch will partner Jermain Defoe up front in McClaren's first game in charge of the national team.
The starting line-up has a slightly unfamiliar look to it, with Wayne Rooney, Michael Owen and Joe Cole out injured and David Beckham dropped.
Liverpool midfielder Steven Gerrard will take Beckham's place on the right of midfield, with Middlesbrough's Stewart Downing on the left.
Owen Hargreaves retains his starting place after his impressive showing at the World Cup and will partner Frank Lampard in central midfield.
England team to face Greece:
Paul Robinson; Gary Neville, Ashley Cole, John Terry (capt), Rio Ferdinand; Steven Gerrard, Owen Hargreaves, Frank Lampard, Stewart Downing; Peter Crouch, Jermain Defoe.