Chambers thinks the international break came at the perfect time for Rovers after they claimed a win over then-leaders Cardiff, following a mediocre start to the campaign.
The former Leicester defender says the team are now fit and raring to come away with something from the Madejski Stadium against a Reading side who are having a less-than-stellar season themselves.
"It has been a busy start and we've been playing two games a week since the start of the season so the break came at the right time," Chambers told the Sheffield Star.
"It was good to go into the break with a win over Cardiff. Prior to that we'd be playing well but not getting the results that we probably deserved.
"Reading haven't made the best of starts, which surprises me, but they brought in a few new players at the start of the season and they are probably still trying to gel, so we've got to try and maintain our momentum and pick up some sort of result."