Dagenham manager John Still has doubts over defenders Scott Doe and Abu Ogogo ahead of the momentous trip to Hillsborough.
Doe picked up a calf injury in the pre-season game at Braintree last weekend while full-back Ogogo suffered a hip injury in the same match.
Both players have a chance of playing the biggest game in the club`s history and will be assessed before kick-off.
Striker Paul Benson (ankle) is likely to start up front despite being linked with a move to Charlton with new signing Gavin Tomlin likely to partner him on his debut.
The Daggers have never played in the third tier of league football before and also never faced the Owls in a league fixture.