Colchester will check on Magnus Okuonghae and Anthony Wordsworth ahead of the visit of Leyton Orient.
Centre-half Okuonghae (ankle) and winger Wordsworth (hamstring) were ruled out of Saturday's 2-1 win over Bournemouth which lifted the Us up to third.
Defender Pat Baldwin is pushing to be added to the squad after recovering from a foot injury, but winger Simon Hackney is still on the sidelines with a back problem and striker Steven Gillespie misses out with a thigh strain.
Tommy Williams plays the final game of his loan spell from Bristol City, with fit-again regular left-back Marc Tierney (shoulder) on the bench again.
Williams crashed in a 30-yard free-kick, his first goal in six years, against the Cherries.