Rooney has recovered from a minor hamstring strain while central defensive duo Nemanja Vidic and Rio Ferdinand are also set to be reinstated to the starting XI having been rested for Wednesday's Champions League win over Schalke.
Fired up: Rooney will lead United's attack against Chelsea
Darren Fletcher will not start after his recent virus, with United needing to avoid defeat to retain top spot with two games remaining.
Team (from): Van der Sar, Kuszczak, Lindegaard, Rafael, O'Shea, Ferdinand, Vidic, Smalling, Evans, Brown, Evra, Valencia, Scholes, Anderson, Carrick, Fletcher, Gibson, Park, Giggs, Nani, Obertan, Rooney, Berbatov, Owen, Hernandez.
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Explore more:People: Wayne Rooney, Darren Fletcher, Rio Ferdinand, Nemanja Vidic, Nani