The injury-hit midfielder has not travelled to Lancashire and his absence will serve as a disappointing blow to boss Carlo Ancelotti who had hoped the England international would be available at the Reebok Stadium.
But Lampard will now target this weekend's FA Cup fourth round clash against Everton for his comeback after failing to train for most of last week with the minor injury problem
Missing man: Frank Lampard has failed to recover in time from a minor calf injury
The 32-year-old has suffered a frustrating season so far, spent mostly on the sidelines. He returned to action last month following a lengthy spell out.
Ancelotti is confident that Lampard, who has made just 11 appearancesfor the Blues this term, can still play an integral part in his side's quest for honours.
He said last week: 'We were without him for some months and knew it was very important to have him back. I still think that Lampard is readyfor a fantastic season.'
Champions Chelsea are ten points behind league leaders Manchester United in fourth place and a win over Bolton would see them close the gap on third-placed Manchester City to fourth points.
Frustration: Injury-hit Lampard has spent most of the season on the sidelines
Lampard, speaking at the weekend, insists Chelsea face a true test ofcharacter in their forthcoming games - with three consecutive matches away from Stamford Bridge.
'With Everton in the FA Cup thrown in between the Bolton and Sunderland games, it is a big period of away games and it is a test of strength and character to go up north,' he told the club's official website.
'The league games are evening games as well and it will be a chance to show that we are on our way back because we need to keep the run going, not that it is a long run of wins yet. We need to make it a long run of wins.'
Captain John Terry, who had been a doubt with a slight back problem, did make the journey to the north-west, Chelsea confirmed this morning on their club website.
Chelsea ponder move for Simon Kjaer after £26m bid for David Luiz stallsRoman's army must storm the Reebok in order to retain Premier League title MARTIN SAMUEL: Roman Abramovich's power play has insulted a good manAll the latest Chelsea news, features and opinion
Explore more:People: Frank Lampard, Carlo Ancelotti, John Terry Places: Bolton, United Kingdom