Chelsea left-back Ashley Cole has been diagnosed with a depressed fracture of the tibia which is likely to keep him out of action for several weeks.
The 28-year-old aggravated the problem before Sunday's vital victory against Manchester United, although he did manage to play in the 1-0 win.
He will definitely play no part in England's friendly international against Brazil on Saturday.
The defender missed three games after initially sustaining the injury in the Blues' 4-0 home win against Atletico Madrid in the Champions League last month.
He was sidelined for a fortnight but was able to play in the return fixture in the Spanish capital last week, a game that eventually finished 2-2.
A spokesman for Chelsea confirmed: "Ashley Cole has not been selected in the England squad for their friendly against Brazil.
"He has a depressed fracture of the tibia, an injury first picked up in the Atletico Madrid home match last month.
"He aggravated the injury before the match against Manchester United and required an injection to play.
"However, he further injured it during the game and has therefore been ruled out of this week's England squad."