England left-back Cole will start on the bench against Spurs despite having no comeback matches since breaking his ankle against Everton earlier this season.
His return is also a massive boost for England boss Fabio Capello ahead of the World Cup finals in South Africa this summer.
Chelsea coach Carlo Ancelotti said: "Ashley has not played but he is very fit and trained all week with us.
"He will be on the bench tomorrow."
Ancelotti will recall also Deco, Joe Cole and Florent Malouda after all three were left out of the starting line-up for the 1-0 win over Bolton on Tuesday night.
Michael Essien (knee), Ricardo Carvalho (ankle) and Jose Bosingwa (knee) are all sidelined.
Kick-off at White Hart Lane is 5:30pm.
Chelsea (from): Cech, Hilario, Turnbull, A Cole, Kalou, Alex, Drogba, Malouda, Terry, Borini, Lampard, Obi, Deco, Anelka, Zhirkov, J Cole, Belletti, Ballack, Kakuta, Sturridge, Ferreira, Bruma, Ivanovic.